Preparation and Biomedical properties of transparent chitosan/gelatin/honey/aloe vera nanocomposite

Objective(s): Biodegradable polymers are featured with notable potentials for biotechnology and bioengineering purposes. Still, there are limitations in their applicability so that in many cases composite forms are used. The present study is focused on chitosan (CS), gelatin (GEL), honey (H) and aloe vera (AV) for preparation of thin films.Methods: To prepare the thin film, CS and GEL with ratio of 8 % was used. Similarly, AV, honey and wheat germ oil (WGO) was prepared with 20:20:2 ratio. Afterward, the solution was poured in petri dishes. The dishes were stored at room temperature for 24h until the film was formed. Then, CS/PEO/H, CS/PEO/AV, CS/PEO/H/AV nanofibers electrospinning was done on CS/GEL/H, CS/GEL/AV, CS/GEL/H/AV thin films, respectively. Results: The results of antibacterial activity, fibroblast cells culture and hemolytic activity were examined. The results of antibacterial tests revealed that thin films containing honey had antibacterial activity against staphylococcus aureus and pseudomonas aeruginosa. The results of fibroblast cells on the prepared samples indicated more than 90% of the cells are alive. Also,hemolytic activity results indicate that the samples are non-hemolytic.Conclusions: Considering the overall physiochemical and biological properties of the thin films, they could be advantageously used as a promising biomaterial for antibacterial coating.